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Producing the engineered viruses that carry a CAR gene into T cells. Viral vector manufacturing is a long-standing bottleneck for cell and gene therapy.
Lentiviral vectors for CAR-T are made by transient transfection of HEK293 cells with plasmid DNA, or increasingly by stable producer cell lines; AAV serves in vivo gene therapy. Capacity shortages in 2018-2022 delayed trials; large CDMOs (Lonza, Thermo Fisher, Charles River, Oxford Biomedica) and in-house plants (Kite, Novartis, BMS) have since expanded. Titre, empty-capsid ratio, and cost per dose are the quality and economic levers.
Packaging and transfer plasmids co-transfected into producer cells; harvested particles are purified by chromatography and tested for titre, potency, and replication competence.
Dependencies are what this technology cannot be delivered without: manufacturing steps, instruments, software, upstream methods. See its full chain on the map.
Query for this technology: (TITLE:"Viral vector manufacturing" OR ABSTRACT:"Viral vector manufacturing" OR TITLE:"lentiviral, retroviral, AAV" OR ABSTRACT:"lentiviral, retroviral, AAV") AND (cancer OR tumor OR tumour OR oncology OR carcinoma OR lymphoma OR leukemia OR leukaemia OR myeloma OR sarcoma OR melanoma OR glioma). Results are unfiltered search hits about Viral vector manufacturing (lentiviral, retroviral, AAV), not a curated reading list.
Shares Charles River Laboratories, Allogeneic cell banks: one donor, hundreds of doses, Autologous CAR-T manufacturing, batch by batch, CAR-NK & CAR-macrophage and the tag supporting.
Shares Catalent (Novo Holdings), CDMO (contract development and manufacturing organisation), Thermo Fisher Scientific, Lonza and the tag supporting.
Shares Cytiva (Danaher), Single-use bioprocessing systems, Lonza, Autologous CAR-T manufacturing, batch by batch and the tag supporting.
Shares Allogeneic cell banks: one donor, hundreds of doses, Autologous CAR-T manufacturing, batch by batch, TCR-T cell therapy, CAR-T cell therapy and the tag supporting.
Shares Charles River Laboratories, Thermo Fisher Scientific and the tag supporting.
Shares Plasmid DNA and mRNA raw-material manufacturing and the tag supporting.
Shares Plasmid DNA and mRNA raw-material manufacturing and the tag supporting.
Shares CDMO (contract development and manufacturing organisation), Lonza and the tag supporting.