Loading
A way to stain a tumour slice for dozens of proteins at once using metal-tagged antibodies read by a mass spectrometer, giving a detailed map of which cells are where.
Imaging mass cytometry (Standard BioTools Hyperion) and multiplexed ion beam imaging (MIBI) label antibodies with rare-earth metal isotopes instead of fluorophores, then ablate the tissue pixel by pixel and read the metals by mass spectrometry. Forty or more markers can be measured on one section without spectral overlap, letting researchers phenotype immune and tumour cells in place and quantify their spatial relationships.
Metal-isotope-tagged antibodies bind the section; a laser or ion beam vaporises each pixel and time-of-flight mass spectrometry counts the isotopes, building one image per marker.
Query for this technology: (TITLE:"Imaging mass cytometry" OR ABSTRACT:"Imaging mass cytometry") AND (cancer OR tumor OR tumour OR oncology OR carcinoma OR lymphoma OR leukemia OR leukaemia OR myeloma OR sarcoma OR melanoma OR glioma). Results are unfiltered search hits about Imaging mass cytometry, not a curated reading list.
Open-source projects that implement or serve this technology, from OnCo's own catalogue: licence and last activity as the repository reported them on the day of the fetch. Listing is not endorsement; check the licence before reuse and the validation before clinical use.
Harvard's pipeline for multiplexed tissue imaging: stitching, segmentation and single-cell quantification for CyCIF, CODEX and similar data.